论文部分内容阅读
David.E.L等人将带有亲电基团的芴衍生物作了Ames试验,并指出2,7—二硝基芴和2,4,7—三硝基—9—芴酮可能有致癌作用。对芴的致突变机理进行了探讨。作者用沙门氏伤寒杆菌的变种TA100(携带替代突变hisG46),TA1537(带有组氨酸移码突变his3076),TA1538以及TA98。上述四个菌种都是脂多糖屏障(rfa)和紫外线修复(uvrB)双重缺陷型菌株。阳性对照用10％甲基甲磺酸酯,9—氨基吖啶500微克/毫升,2—硝基芴250微克/毫升,柔毛霉素250微克/毫升,与芴的衍生物同样操
David.EL et al. Conducted Ames test on fluorene derivatives with electrophilic groups and pointed out that 2,7-dinitrofluorene and 2,4,7-trinitro-9-fluorenone may have carcinogenic effects . The mutagenic mechanism of fluorene was discussed. The authors used the Salmonella typhimurium variant TA100 (carrying the substitution mutation hisG46), TA1537 (histidine frameshift mutation his3076), TA1538 and TA98. The above four strains are both lipopolysaccharide barrier (rfa) and UV-repair (uvrB) double-deficient strains. The positive control was treated with 10% methyl methanesulfonate, 500 μg / ml 9-aminoacridine, 250 μg / ml 2-nitrofluorene and 250 μg / ml daunorubicin