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目的探讨体外受精-胚胎移植(in vitro fertilization-embryo transfer,IVF-ET)患者卵巢颗粒细胞中环氧合酶-2(cyclooxygenase-2,COX-2)的表达,分析其与妊娠结局的关系。方法荧光半定量real-time PCR检测48例行IVF-ET患者卵巢颗粒细胞中COX-2的mRNA表达,分析COX-2与注射hCG日血清雌二醇(estradiol,E_2)水平、获卵率、受精率、优质胚胎率及妊娠率的关系。结果卵巢颗粒细胞COX-2的表达量与hCG日血清E_2水平及获卵率无关,与受精率、优质胚胎率及妊娠率相关。结论 PCOS患者卵巢颗粒细胞COX-2的表达与胚胎质量相关,研究卵巢颗粒细胞的COX-2的表达可以作为预测IVF-ET妊娠结局指标之一。
Objective To investigate the expression of cyclooxygenase-2 (COX-2) in ovarian granulosa cells from patients with in vitro fertilization-embryo transfer (IVF-ET) and to analyze its relationship with pregnancy outcome. Methods The mRNA expression of COX-2 in ovarian granulosa cells of 48 patients with IVF-ET was detected by semi-quantitative real-time PCR. The levels of COX-2 and estradiol (E 2) Fertilization rate, quality embryo rate and pregnancy rate. Results The expression level of COX-2 in ovarian granulosa cells was not related to the level of serum E_2 and the ovulation rate on hCG day, and was related to fertilization rate, high quality embryo rate and pregnancy rate. Conclusions The expression of COX-2 in ovarian granulosa cells of PCOS patients is related to the quality of embryo. To study the expression of COX-2 in ovarian granulosa cells can be used as one of the indexes to predict the outcome of IVF-ET.