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目的考察阿魏菇多糖(pleurotus ferulae polysaccharide,PFP)对四氯化碳(CCl4)致小鼠急性肝损伤的保护作用。方法制备CCl4小鼠急性肝损伤模型,采用阿魏菇多糖(125、250和500 mg·kg-1)灌胃给药,利用试剂盒检测小鼠血清丙氨酸转氨酶(alanine aminotransferase,ALT)和天冬氨酸转氨酶(aspartate aminotransferase,AST)活性,检测肝组织中丙二醛(malondialdehyde,MDA)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)和超氧化物歧化酶(superoxide dismutase,SOD)的活力。HE染色观察肝组织病理学变化。结果阿魏菇多糖能够降低模型小鼠血清AST和ALT含量,增强肝组织SOD和GSH活力,降低肝组织MDA水平,显著改善肝组织结构,减少肝细胞坏死。结论阿魏菇多糖对CCl4致小鼠急性肝损伤有一定的保护作用。
Objective To investigate the protective effect of pleurotus ferulae polysaccharide (PFP) on acute liver injury induced by carbon tetrachloride (CCl4) in mice. Methods Acute hepatic injury model was induced in CCl4 mice. Administration of the polysaccharide of Pleurotus ostreatus (125, 250 and 500 mg · kg-1) intragastrically to the mice was used to detect the serum levels of alanine aminotransferase (ALT) and The activity of aspartate aminotransferase (AST) was measured. The levels of malondialdehyde (MDA), glutathione peroxidase (GSH-Px) and superoxide dismutase, SOD) vitality. Hepatic histopathological changes were observed by HE staining. Results Fermented with Pleurotus ferulae could decrease the levels of AST and ALT, increase the activities of SOD and GSH, decrease the level of MDA in liver tissue, improve the structure of liver tissue and reduce the necrosis of liver cells. Conclusion The polysaccharide of Pleurotus ostreatus has certain protective effect on acute liver injury induced by CCl4 in mice.