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用浓度自12.5～3200μM成倍递增的氯喹、奎宁或甲氟喹水溶液,按每井25μl加入培养板各井内,井径为16mm。干燥后置于15～25℃暗室备用。每种药物浓度测3次,并设对照井。培养用RPMI 1640液,以25mM/l的HEPES及20mM/l的NaHCO_3缓冲,并加10%新鲜正常人血清,经孔径为0.22μm滤膜过滤后贮于-25℃备用。含虫红细胞获自疟疾患者或培养株,存于4℃下不超过72小时,健康的红细胞用缓冲的RPMI洗5次,离心(1500rpm)后移去上端的白细胞和血小板,配成含红细胞5%的悬液再添
With a concentration of 12.5 ~ 3200μM doubled the increase of chloroquine, quinine or mefloquine aqueous solution, 25μl per well added to the wells of the well, the caliber of 16mm. After drying placed in a dark room at 15 ~ 25 ℃ spare. Each drug concentration measured 3 times, and set control wells. Cultures were prepared with RPMI 1640 buffer, buffered with 25 mM HEPES and 20 mM NaHCO 3, supplemented with 10% fresh normal human serum, filtered through a 0.22 μm membrane filter and stored at -25 ° C until use. The worm-containing erythrocytes were obtained from malaria patients or cultured strains and stored at 4 ° C for no more than 72 hours. Healthy red blood cells were washed 5 times with buffered RPMI and centrifuged (1500 rpm), then the upper white blood cells and platelets were removed to make erythrocytes 5 % Of the suspension to add