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本文报告了在沙特阿拉伯阿赫萨绿洲对动物利什曼病的调查结果。在绿洲7个地点的鼠洞口安放以嗜盐植物和麦类种子为诱饵的捕鼠器捕捉活的肥大沙鼠(Psammomys obesus)和利比亚沙鼠(Meriones lybycus)。将捕获的鼠立即送检,检查鼠体表损害,取损伤边缘组织和耳部皮肤组织,制成涂片,用姬氏液染色后镜检利什曼原虫。将整只鼠用含去污剂的水充分洗净后用消毒液处理,然后取耳组织,内脏和心脏血,按Herre等(1966)方法用改良的托比(Tobie)琼脂基培养,并将
This paper reports the findings of an investigation into animal leishmaniasis in Ahsa Oasis, Saudi Arabia. Survival of Psammomys obesus and Meriones lybycus were captured with mouse traps baited with halophilic plants and wheat seeds in rat holes in seven oasis locations. The captured mice were immediately seized, check the damage to the rat body surface, take the damaged marginal tissue and ear skin tissue, made smear, microscopy Leishmania stain with Ji’s solution. The whole rat was thoroughly washed with a detergent-containing water and then treated with a disinfectant solution. Ear tissues, viscera, and heart blood were collected and cultured using a modified Tobie agar based on the method of Herre et al. (1966) will