论文部分内容阅读
目的:寻找一种快速简便的奶粉中坂崎肠杆菌的检测方法。方法:通过对不同增菌液及分离平板的试验比较,选择对坂崎肠杆菌的检测适宜的预增菌液及分离平板。结果:通过参考菌株的添加试验,灭菌蒸馏水和缓冲蛋白胨水作为检测的预增菌液,增菌效果差异没有显著性,将34株肠杆菌科细菌的参考菌株分别划线接种于显色培养基琼脂(Xα-G lcA)、山梨醇麦康凯琼脂(SMAC)、四甲基伞形酮培养基(NA+α-MUG)和结晶紫中性红胆盐葡萄糖琼脂(VRB-GA),选择性分离效果最好的是Xα-G lcA。结论:推荐使用无菌蒸馏水作为检测的预增菌液,Xα-G lcA或VRBGA为分离平板,API 20E生化试剂盒或VITEK进行生化鉴定。
Objective: To find a quick and easy method for the determination of Enterobacter sakazakii in milk powder. Methods: By comparing different enrichment broth and separating plate, we chose the appropriate pre-broth and separation plate for the detection of Enterobacter sakazakii. Results: The results of reference strains addition, sterilized distilled water and buffered peptone were used as pre-enrichment broth. There was no significant difference in the bacteriostasis between the strains. Reference strains of 34 strains of Enterobacteriaceae were streaked in the color culture (AgC), SMAC, NA + α-MUG and VRB-GA were selected, Sexual separation is the best Xα-G lcA. CONCLUSIONS: Sterile distilled water is the recommended pre-boosted solution. Xα-GlcA or VRBGA is biochemically identified on plates, API 20E biochemical kits or VITEK.