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以20%聚乙二醇(PEG6000)处理模拟干旱,利用一氧化氮(nitric oxide,NO)供体硝普钠(sodium nitroprusside,SNP)处理平邑甜茶[Malus hupehensis(Pamp.)Rehd.]幼苗,探讨外源NO对水分胁迫下平邑甜茶幼苗叶绿素荧光参数和光合速率的影响。结果表明:水分胁迫下平邑甜茶幼苗Fo显著上升,Fv/Fm、qP、ETR、Yield、叶绿素含量和光合速率显著降低,qN在胁迫的前3d呈上升趋势,之后大幅度下降;通过外施不同浓度的SNP(100~700μmol·L-1)均使水分胁迫下平邑甜茶幼苗Fo上升及Fv/Fm、ETR、qP、Yield、叶绿素含量和光合速率下降幅度明显减小。不同处理缓解作用为:300μmol·L-1SNP>500μmol·L-1SNP>100μmol·L-1SNP>700μmol·L-1SNP,其中300~500μmol·L-1SNP处理缓解效应明显,但以300μmol·L-1SNP处理效果最好。
The simulated drought was treated with 20% polyethylene glycol (PEG 6000) and the seedlings of Malus hupehensis (Pamp.) Rehd. Were treated with nitric oxide (NO) donor sodium nitroprusside (SNP) , To explore the effects of exogenous nitric oxide on chlorophyll fluorescence parameters and photosynthetic rate of Malus hupehensis Rehd. Under water stress. The results showed that under the water stress, the Fo of the seedlings of P. euphorbiae increased significantly, the content of Fv / Fm, qP, ETR, Yield, chlorophyll and photosynthetic rate decreased significantly, qN increased in the first three days of stress and then dropped significantly; The concentration of SNP (100 ~ 700μmol·L-1) decreased the Fo and decreased the Fv / Fm, ETR, qP, Yield, chlorophyll content and photosynthetic rate of P. euphratica seedlings under water stress. The effect of different treatments was as follows: 300μmol·L-1 SNP> 500μmol·L-1 SNP> 100μmol·L-1 SNP> 700μmol·L-1SNP, of which 300 ~ 500μmol·L-1 SNP had a significant relieving effect. The best treatment.