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采用体外抗体应答系统及溶血空斑检测技术研究了4种常用的中药注射液、4种有代表性的提纯中药成分及一种化学合成药——羧甲基淀粉(CMS,代号404)—的免疫调节效用。结果证明,>0.05％的板兰根及益母草注射液有免疫抑制效用,而0.0005％板兰根有免疫增强作用,皆较小影响细胞活力;>0.05％丹参虽有免疫抑制作用,但影响细胞活力也较著;龙胆草的免疫抑制作用较弱,且影响细胞活力也较著。在提纯的中药中,芒果甙(0.1μg/ml)有免疫抑制效用而不影响细胞活力;大黄素葡萄糖苷及丹皮酚的免疫抑制皆较弱,且影响细胞活力;刺五加多糖(PES)在高浓度时有免疫抑制效用,而在低浓度时则有显著免疫增强作用,並不影响细胞活力。羧基甲淀粉在0.01～500μg/ml中皆有显著免疫增强作用而不影响细胞活力。此实验还证明体外诱生抗体必须有粘附细胞参与。文内讨论了此法适用性及优缺点。
The use of in vitro antibody response system and hemolytic plaque detection technology to study the four commonly used traditional Chinese medicine injections, four representative purification of traditional Chinese medicine ingredients and a chemical synthesis drug - carboxymethyl starch (CMS, code 404) - Immunomodulatory effects. The results showed that >0.05% of Banlangen and motherwort injections had immunosuppressive effects, while 0.0005% Banlangen had immune-enhancing effects, which all had less effect on cell viability; >0.05% Salvia miltiorrhiza, although it had immunosuppressive effects, but also had significant effects on cell viability. The immunosuppressive effect of Gentian is weak, and it affects the cell viability as well. In the purified Chinese medicine, mangoquinone (0.1μg/ml) has immunosuppressive effect without affecting cell viability; emodin glucoside and paeonol have weaker immunosuppression and affect cell viability; Acanthopanax Senticosus polysaccharide (PES) ) Immunosuppressive effect at high concentrations and significant immunoenhancement at low concentrations does not affect cell viability. The carboxymethyl starch had significant immunopotentiation in 0.01-500 μg/ml without affecting cell viability. This experiment also demonstrated that in vitro induced antibodies must have adherent cells involved. The applicability and advantages and disadvantages of this law are discussed in the article.