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目的 构建编码抗人精浆蛋白抗体Fab基因的表达载体 ,并在大肠杆菌中进行表达 .方法 :从克隆载体pUC1 9 K和 pBlue scriptKS (M1 3) Fd中 ,酶切获得抗人精浆蛋白单克隆抗体 (mAb)Fd基因和κ链基因 .然后将Fd和κ链基因重组到Fab表达载体pComb3中 ,构建抗人精浆蛋白Fab基因的重
OBJECTIVE: To construct an expression vector encoding the Fab gene of anti-human seminal plasma protein antibody and express it in E.coli.Methods: Anti-human seminal plasma proteinase was obtained by digestion with cloning vector pUC19K and pBlue scriptKS (M1 3) Fd (MAb) Fd gene and kappa chain gene, and then the Fd and kappa chain genes were recombined into the Fab expression vector pComb3 to construct an anti-human seminal plasma protein Fab gene