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研究了用透析平衡法制备碱性蛋白酶晶体的过程，在硫酸铵饱和度为２５％的条件下获得了符合要求的碱性蛋白酶晶体，然后采用戊二醛进行交联，制备出了交联酶晶体，并对其在高酸碱度、高温、有机溶剂或有机溶液中的稳定性进行了研究．实验结果表明，交联酶晶体有较高的稳定性．
The process of preparation of alkaline protease crystals by dialysis equilibrium method was studied. Alkaline protease crystals with satisfactory ammonium sulfate saturation of 25% were obtained and then crosslinked with glutaraldehyde to prepare cross-linked enzyme Crystal, and its stability in high pH, high temperature, organic solvent or organic solution was studied. The experimental results show that cross-linked enzyme crystals have high stability.