论文部分内容阅读
【目的】探讨转录因子 SOX2表达下调对宫颈癌细胞增殖及侵袭能力的影响。【方法】将特异性短发夹（shRNA）SOX2干扰载体转染宫颈鳞癌细胞 SiHa获得稳转细胞系（SOX2干扰组），MTT法检测细胞增殖能力变化，Transwell小室法测定细胞侵袭能力差异，Western Blot检测 SOX2、MMP2、MMP9及上皮间质转化相关因子变化，并与阴性对照转染组与空白对照组比较。【结果】与阴性对照转染组和空白对照组比较， SOX2干扰组 MTT结果显示细胞增殖能力明显降低，Transwell结果显示细胞穿膜数显著减少、侵袭能力降低（P＜0．05），Western Blot检测 SOX2显著下调、MMP2、MMP9表达下调、E-cadherin蛋白水平升高、Vim-entin表达降低（P＜0．05）；而阴性对照转染组与空白对照组相比各项指标无明显变化。【结论】抑制 SOX2表达能够抑制宫颈鳞癌细胞的体外增殖与侵袭能力，可能与抑制上皮间质转化过程有关。“,”[Obj ective]To explore the effect of the up-regulation of transcription factor SOX2 on cell prolif-eration and invasion of cervical cancer cells.[Methods]The specific short hairpin(shRNA)SOX2 interference vector was transfected into cervical squamous cell SiHa in order to obtain stable transfection cell line(SOX2 in-terference group).MTT assay was used to detect the change of cell proliferation.The difference of cell inva-sion was detected by using Transwell invasion cabin.Western Blot was used to detect the expressions of SOX2,MMP2,MMP9 and epithelial-mesenchymal transition associated factors,which were compared with those in negative transfection control group and blank control group.[Results]MTT result showed that cell proliferation in SOX2 interference group was significantly reduced in comparison with negative transfection control group and blank control group.Transwell result showed that cell-penetrating number was significantly reduced and cell invasion was decreased(P<0.05).Western Blot result showed that SOX2 was up regulated markedly,and the expressions of MMP2,MMP9 were decreased,but the protein level of E-cadherin was in-creased and the expression of Vimentin was decreased(P<0.05).There was no significant difference in all indicators between negative transfection control and blank control group.[Conclusion]Suppression of SOX2 expression can effectively inhibit proliferation and invasion of cervical cancer cell in vitro,which may be related to the inhibition of epithelial-mesenchymal transition processes.