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采用网袋法在室内模拟研究了Bt(Bacillus thuringiensis)玉米34B24和1246×1482秸秆地表覆盖还田淹水和非淹水条件下Bt蛋白的降解动态,并应用移动对数模型进行拟合及估算DT50和DT90,探讨淹水对Bt玉米秸秆地表覆盖下Bt蛋白降解过程的影响。结果表明:两品种秸秆中Bt蛋白在室内模拟地表覆盖淹水和非淹水条件下均能快速降解,降解动态均呈前期快速大量降解和中后期极低量稳定下降两个阶段,1周内两品种秸秆淹水和非淹水条件下Bt蛋白均已降解了90%以上,但到180 d时,还可检测到占初始含量0.009%~0.017%的Bt蛋白。Bt蛋白ELISA法实测数据和移动对数模型拟合得出的DT50和DT90均表明非淹水条件下Bt蛋白降解较慢,但淹水促进秸秆中Bt蛋白降解的作用仅表现在地表覆盖初期(第1 d),其后影响不大。
The net bag method was used to simulate the biodegradation of Bt protein in Bt (Bacillus thuringiensis) maize (Zea mays) 34B24 and 1246 × 1482 strata under flooded and non-flooded conditions, and fitted and calculated by moving logarithm model DT50 and DT90 to investigate the effects of flooding on the degradation of Bt protein under surface-covered Bt corn stover. The results showed that Bt protein in two kinds of straw could rapidly degrade under simulated ground surface submerged conditions and non-submerged conditions. The dynamic degradation of Bt protein in two kinds of straw showed rapid degradation in early prophase and steady decline in very low mid- Bt protein degraded more than 90% under both flooded and non-flooded conditions, but Bt protein with the initial content of 0.009% -0.017% could be detected by 180 d. The DT50 and DT90 obtained by the Bt protein ELISA and the log-migration model both showed that the degradation of Bt protein was slower in non-flooded condition, but the effect of flooding on the degradation of Bt protein in straw was only observed in the early stage of surface coverage 1 d), then little effect.