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采用３Ｄ－ＨＰＬＣ对胃脘舒冲剂进行了定性鉴别并建立其指标性成分的定量分析方法。色谱柱：ＣＬＣ－ＯＤＳ（６．０ ｍｍ × １５０ ｍｍ，５ μｍ）；流动相：乙腈－０．１％醋酸水溶液系统；洗脱方式：二元高压梯度洗脱；检测方式；二级管阵列检测器多通过检测；测定波长：芍药苷２３０ ｎｍ，甘草酸２５４ｎｍ，授应苷 ２８３ ｎｍ；各指标性成分的线性范围分别为：芍药苷 ４．２３～６７．６８ μｇ／ｍＬ（ｒ＝０．９９９９，ｎ＝５），橙皮苷 ７．５０～１１９．２０ μｇ／ｍＬ（ｒ＝０．９９９８，ｎ＝５），甘草酸 １０．５０～１６８．００μｇ／ｍＬ（ｒ＝０．９９９４，ｎ＝５）；加样回收率及ＲＳＤ分别为：芍药苷８９．４６％，２．８７％，橙皮苷９３．７６％，２．４８％，甘草酸９０．２２％，１．７９％。同时用三维ＨＰＬＣ图谱对胃脘舒冲剂进行了定性别。
3D-HPLC was used to qualitatively identify Weiqishu granules and establish a quantitative analysis method of its index components. Column: CLC-ODS (6.0 mm × 150 mm, 5 μm); mobile phase: acetonitrile-0.1% acetic acid aqueous solution system; elution method: binary high pressure gradient elution; detection method; diode array The detectors mostly passed the detection; the measurement wavelengths were: paeoniflorin 230 nm, glycyrrhizic acid 254 nm, and adenosine 283 nm; the linear range of each index component was: paeoniflorin 4.23 to 67.68 μg/mL (r=0 .9999, n=5), hesperidin 7.50 to 119.20 μg/mL (r=0.9998, n=5), glycyrrhizic acid 10.50 to 168.00 μg/mL (r=0.9994 , n=5); The recovery of samples and RSD were as follows: paeoniflorin 89.46%, 2.87%, hesperidin 93.76%, 2.48%, glycyrrhizic acid 90.22%, 1.79 %. At the same time, a three-dimensional HPLC profile was used to determine the gender of Weiqishu granules.