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胸腺蛋白(thymus protein)是从健康乳猪新鲜胸腺中提取的具有生物活性的蛋白类水溶液,可用于胃溃疡和十二指肠溃疡的治疗。近年来,该产品在临床上越来越受到重视,但目前尚无生物活性的检测方法。本实验应用体外培养小鼠胚胎成纤维细胞(BALB/c 3T3),采用MTT[1]法检测胸腺蛋白口服液(修正药业集团北京修正制药有限公司生产,批号:20120501)促细胞增殖作用,为进一步用于治疗消化系统溃疡提供科学
Thymus protein is a bioactive aqueous solution of protein extracted from the fresh thymus of healthy suckling pigs and can be used for the treatment of gastric ulcer and duodenal ulcer. In recent years, the product is getting more and more attention in the clinic, but there is no biological activity testing method at present. In this study, mouse embryonic fibroblasts (BALB / c 3T3) were cultured in vitro and the thymocyte protein oral solution (ameliorated by Beijing Pharmaceutical Industry Group Co., Ltd., batch number: 20120501) was detected by MTT assay [1] To further for the treatment of digestive ulcer to provide science