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目的:研究以CD45/CD138设门检测多发性骨髓瘤细胞的方法。方法:将35例多发性骨髓瘤患者以骨髓瘤细胞/有核细胞>10%或<10%分为瘤细胞高比例组和低比例组,以CD45/CD138或CD45/SS两种方法设门检测两组患者的免疫表型,对两种设门方法进行比较。结果:两种方法设门检测,高比例组、低比例组免疫表型相近:瘤细胞主要阳性检出CD138、CD38、CD56,阴性检出CD10、CD19、CD20、CD22、CD33、CD34、CD2、CD3、CD25、CD5、CD7、HLA-DR、CD11c。但两种方法比较:前者目标细胞群/有核细胞比值显著性低于后者,CD38及CD56抗原阳性检测值显著性高于后者;瘤细胞高比例组CD38、CD56检出率显著性高于低比例组。以CD45/CD138设门,除了能区分骨髓瘤细胞群,还能区分浆细胞群。结论:CD45/CD138较CD45/SS更适合设门用于多发性骨髓瘤的诊断和微小残留病的检测;骨髓瘤细胞未见异质性抗原表达;CD/CD设门,可以区别骨髓瘤细胞群和成熟浆细胞群。
Objective: To study the method of detecting multiple myeloma cells by CD45 / CD138. Methods: Thirty-five patients with multiple myeloma were divided into high and low proportion of myeloma cells / nucleated cells> 10% or <10% with CD45 / CD138 or CD45 / SS The immunophenotypes of two groups of patients were tested and the two methods of gating were compared. Results: The two methods were phylogenetically tested. The immunophenotypes were similar in the high-proportion group and the low-proportion group. The tumor cells were mainly positive for CD138, CD38 and CD56, negative for CD10, CD19, CD20, CD22, CD33, CD34, CD2, CD3, CD25, CD5, CD7, HLA-DR, CD11c. However, the two methods were compared: the former target cell population / nucleated cell ratio was significantly lower than the latter, CD38 and CD56 antigen positive test was significantly higher than the latter; high proportion of tumor cells CD38, CD56 detection rate was significantly higher In low proportion group. With CD45 / CD138 door, in addition to differentiate myeloma cell population, but also to distinguish between plasma cells. Conclusion: CD45 / CD138 is more suitable for the diagnosis of multiple myeloma and detection of minimal residual disease than CD45 / SS. No heterogeneous antigen expression is found in myeloma cells. CD / CD docking can differentiate myeloma cells Population and mature plasma cell populations.