First Isolation and Identification of β-Sitosterol and Kaempferol-3-O-β-D-glucoside from RHIZOME CHI

• 出 处: Medicinal Plant
• 发表日期: 2012年01期
• 关键词: glucoside kaempferol sitosterol silica elucidated isolate convenient dissolved p

[Objective]To study the chemical constituents of RHIZOME CHINESE UMBRELLALEAF in Taibai Mountain. [Method]The chemical constituents were isolated and purified by silica gel column chromatography,TLC and recrystallization. Their structures were elucidated on the basis of physicochemical properties and spectra analysis. [Result]Six chemical compounds were obtained and identified,which were β-sitosterol,podophyllotoxin,podophyllotoxone,kaempferol,diphyllin,kaempferol-3-O-β-D-glucoside. Of them,β-sitosterol and kaempferol-3-O-β-D-glucoside were isolated from this plant for the first time. [Conclusion]This method is simple,convenient,rapid and accurate to isolate and identify the chemical constituents from RHIZOME CHINESE UMBRELLALEAF in Taibai Mountain.

[Method] The chemical constituents were isolated and purified by silica gel column chromatography, TLC and recrystallization. Their structures were elucidated on the basis of physicochemical properties and spectra analysis. [Objective] To study the chemical constituents of RHIZOME CHINESE UMBRELLALEAF in Taibai Mountain. Among them, β-sitosterol and kaempferol-3-O- β-D-glucoside were isolated from this plant for the first time. [Conclusion] This method is simple, convenient, rapid and accurate to isolate and identify the chemical constituents from RHIZOME CHINESE UMBRELLALEAF in Taibai Mountain.