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1970年Temin和Baltimore发现在劳氏鸡肉瘤病毒(RSV)存在依赖于RNA的DNA聚合酶(RD-DP),即反向转录酶,从而修正了遗传信息流的“中心法则”,尔后又在病毒或其他生物中发现逆转录酶。本文作者应用Lewis的方法,成功地在哺乳动物大白鼠的肝脏中分离出RDDP,并对其特征进行了生化分析。将Wistar大白鼠肝脏置于甘氨酸缓冲液中并使之匀浆化,离心得到上清液部分(100,000g)然后用Lewis法分离鼠肝中RDDP,据Lewis的资料,反向转录酶和DNA聚合酶β首先是通过DE-52层析洗脱得到,但在此阶段不能将这两种酶分离开来;因
In 1970, Temin and Baltimore discovered that the presence of RNA-dependent DNA polymerase (RD-DP), the reverse transcriptase, in Rous sarcoma virus (RSV) corrected the “central rule” of genetic information flows and then Reverse transcriptase is found in viruses or other organisms. Using Lewis’ method, the authors successfully isolated RDDP from the liver of mammalian rats and performed biochemical analyzes of their characteristics. The Wistar rat liver was homogenized in a glycine buffer and centrifuged to obtain a supernatant fraction (100,000 g). RDDP was then isolated from the rat liver using the Lewis method. According to Lewis data, reverse transcriptase and DNA polymerizations Enzyme β was first eluted by DE-52 chromatography, but at this stage it was not possible to separate the two enzymes