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目的 探讨创伤后应激障碍 (PTSD)样精神与行为异常的神经生物学机制。方法 在大鼠海马惊厥阈下电刺激PTSD动物模型基础上 ,采用流式细胞仪、荧光标记术及Westernblotting等方法 ,定量观测了PTSD样行为异常大鼠海马细胞内游离Ca2 + 含量与钙调素 (CaM )相对活性平均通道荧光 ,及海马组织总CaM、Ca2 + /CaM依赖性蛋白激酶II偄(CaMKII偄)表达的动态变化规律。结果 电刺激停止后 72h内阈下刺激组实验动物海马细胞内游离钙浓度明显增高 ,游离CaM平均通道荧光则同步降低 ;而海马组织总CaM表达于电刺激停止后 48h内明显增多 ,CaMKII偄表达则显著降低?崧邸『B硐赴鸆a2 + CaM CaMKII偄信号途径调控紊乱可能是实验动物长时程ＰＴＳＤ样行为异?闹匾±砩砘≈
Objective To investigate the neurobiological mechanism of post-traumatic stress disorder (PTSD) -like mental and behavioral abnormalities. Methods Based on the animal model of subthreshold electrical stimulation of hippocampus in rat hippocampus, flow cytometry, fluorescent labeling and Western blotting were used to quantitatively observe the intracellular free Ca2 + levels in hippocampus of PTSD-like rats. (CaM), and the changes of total CaM, Ca2 + / CaM-dependent protein kinase II (CaMKIIII) in hippocampus. Results In the subthreshold stimulation group, the intracellular free calcium concentration in the hippocampus was significantly increased within 72 hours after the termination of electrical stimulation, and the mean fluorescence of free CaM decreased synchronously. However, the total CaM expression in hippocampus significantly increased within 48 hours after the termination of electrical stimulation. The expression of CaMKII 偄Is significantly reduced? Song Di "B to go to Ca a2 CaM CaMKII 偄 signal pathway regulation disorder may be long-term experimental animals PTSD-like behavior of trouble plaque ± 砩 砘 ≈