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为深入探究‘大白谷’(Oryza sativa L.ssp.Indica)感病的分子机理,以及抗病基因在发病过程中所起的作用,克隆了‘大白谷’CC-NBS-LRR蛋白编码基因Os11g RGA8,对Os11g RGA8基因进行了生物信息学分析,并对其在水稻抗干尖线虫侵染过程中的功能进行了研究。Os11g RGA8基因位于11号染色体,其编码蛋白含有733个氨基酸,等电点为5.99,相对分子质量为84355.07。序列分析表明该氨基酸序列含有RX-CC、AAA_22、NBS和LRR_4这4个结构域,Os11g RGA8基因编码抗病蛋白属于CC-NBSLRR抗病蛋白家族。Q-PCR结果显示,接种水稻干尖线虫SAMN02420038的籼型常规稻‘大白谷’,与CK组‘大白谷’相比,Os11g RGA8基因表达量表现为上调,且24 h内上调增幅较缓,48 h时上调增幅突然上涨,72 h时上调增幅又出现下降,表明该基因参与‘大白谷’天然免疫反应过程,在水稻干尖线虫侵染过程中起到一定抗性作用。
In order to further explore the molecular mechanism of susceptibility to Oryza sativa L.ssp.Indica and the role of disease-resistant genes in pathogenesis, we cloned the Os11g gene of CC-NBS-LRR RGA8, the bioinformatics analysis of Os11g RGA8 gene was carried out, and its function in anti-Dorsal root-knot nematode infection in rice was studied. The Os11g RGA8 gene is located on chromosome 11 and encodes a protein of 733 amino acids with an isoelectric point of 5.99 and a relative molecular mass of 84355.07. Sequence analysis showed that the amino acid sequence contained four domains of RX-CC, AAA_22, NBS and LRR_4, and the Os11g RGA8 gene encoded the disease-resistant protein belongs to the CC-NBSLRR resistance protein family. Q-PCR results showed that Os11g RGA8 gene expression was up-regulated compared with CK group “Da Bai Gu”, and the up-regulation of Os11g RGA8 gene expression increased slowly in inoculation with Daheigu, an indica conventional rice with SAMN02420038, At 48 h, the up-regulation increased abruptly, and the up-regulation increased again at 72 h, which showed that the gene was involved in the natural immune response process in Da Bai Valley and played a certain resistance role in the process of A. giganteus infection.