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建立了高效液相色谱法测定拜阿司匹林加维C泡腾片中的2个有效成分(阿司匹林、维生素C)及有关物质(水杨酸、糠醛)。采用Hypersil C18色谱柱,以甲醇∶5 mmol/L四丁基氢氧化铵(以冰乙酸调至pH 4.5)为流动相,梯度洗脱,检测波长247 nm。维生素C、糠醛、阿司匹林和水杨酸分别在24~480、0.5~10、40~800和0.8~16μg/ml浓度范围内线性关系良好,平均回收率分别为100.2%、100.3%、100.2%和99.9%,RSD分别为1.07%、0.85%、0.81%和0.77%。
A HPLC method was established for the determination of two active ingredients (aspirin, vitamin C) and related substances (salicylic acid, furfural) in the aspirin Galve C effervescent tablets. A Hypersil C18 column was used with a mobile phase of methanol: 5 mmol / L tetrabutylammonium hydroxide (adjusted to pH 4.5 with glacial acetic acid) as a mobile phase. The detection wavelength was 247 nm. Vitamin C, furfural, aspirin and salicylic acid showed good linearity in the range of 24 ~ 480, 0.5 ~ 10, 40 ~ 800 and 0.8 ~ 16μg / ml respectively with the average recoveries of 100.2%, 100.3% and 100.2% 99.9% with RSDs of 1.07%, 0.85%, 0.81% and 0.77%, respectively.