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在通常大气压下,日本丽金龟(Popilla japonica Newman)经化学不育剂替派(Tepa)和噻替派(Thiotepa)薰蒸处理后,不论对雄虫或雌虫都产生了绝育效果。其中噻替派更为有效。试验时将替派或噻替派分别溶于几毫升丙酮内(0.75克/试验),然后逐滴滴在直径为15厘米的双层滤纸上,待溶剂挥发后,把它放在容量为4升的玻璃缸内,复以平玻璃板做成薰蒸瓶。另外,从盖子中央小孔中把实验室用的小型搅拌器的轴延伸到瓶内约7厘米左右,以扇动空气,帮助药品挥发。除此之外,其他薰蒸用具和程序都和Borkovec等(1977)介绍的方法相同。试验昆虫除最初试验时是由实验室饲养
At normal atmospheric pressure, Popilla japonica Newman fumigated with the chemical fertilizers Tepa and Thiotepa produced a sterilizing effect on both males and females. Among them, thiotepa is more effective. The test will be sent or thiotepa were dissolved in a few milliliters of acetone (0.75 g / test), and then dripped in double-diameter filter paper 15 cm in diameter until the solvent is volatile, put it in a capacity of 4 Ascension of the glass tank, complex to flat glass made fumigation bottles. In addition, a small agitator shaft for laboratory use was extended from the center hole of the lid to about 7 cm in the bottle to fan the air to help the drug evaporate. In addition, other fumigants and procedures are the same as those described by Borkovec et al. (1977). The test insect was kept in the laboratory except for the initial test