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Objective:To study the influence of MicroRNA-lOb on proliferation and invasion of human low metastatic lung cancer cell 95-C and its mechanism.Methods:Lipofectamine MicroRNA-lOb eukaryotic expression plasmid was transfected into 9S-C.The experiment group was divided into blank control group,empty vector transfected group and MicroRNA-lOb transfected group.Real time quantitative RT-PCR was used to detect the expression of MicroRNA-lOb and KLF4mRNA expression.Proliferations of cells were detected by cell proliferation assay,invasion of the delected the cell Transwell experiments,the expression of KLF4 protein was detected in Western blotting cells.Results:The proliferation rate of MicroRNA-lOb piasmid transfection group increased significantly after transfection,invasion and migration ability enhancement,by comparison,there are statistically significant differences in the blank control group and negative control group(P<0.05);the expression of MicroRNA-lOb piasmid transfection group KLF4protein decreased,the difference was statistically significant(P<0.05);reduce the expression of MicroRNA-l0b piasmid transfection group KLF4mRNA,but no significant differences(P>0.05).Conclusions:MicroRNA-l0b may promote proliferation and invasion of 95-C cells by down regulating the expression of KLF4 protein.
Objective: To study the influence of MicroRNA-lOb on proliferation and invasion of human low metastatic lung cancer cell 95-C and its mechanism. Methods: Lipofectamine MicroRNA-lOb eukaryotic expression plasmid was transfected into 9S-C. The experiment group was divided into blank control group, empty vector transfected group and MicroRNA-lOb transfected group. Real time quantitative RT-PCR was used to detect the expression of MicroRNA-lOb and KLF4 mRNA expression. Proliferations of cells were detected by cell proliferation assay, invasion of the delected the cell Transwell experiments, the expression of KLF4 protein was detected in Western blotting cells. Results: The proliferation rate of MicroRNA-lOb piasmid transfection group increased significantly after transfection, invasion and migration ability enhancement, by comparison, there are statistically significant differences in the blank control group and negative control group (P <0.05); the expression of MicroRNA-lOb piasmid transfection group KLF4 Reduced expression of MicroRNA-l0b piasmid transfection group KLF4 mRNA, but no significant differences (P> 0.05). Conclusions: MicroRNA-l0b may promote proliferation and invasion of 95-C cells by down regulating the expression of KLF4 protein.