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目的:利用人类幽门螺杆菌(H pylori)感染的小鼠模型研究黏附素保守区(AB)疫苗在预防与治疗H pylori感染的作用. 方法:实验分为预防与治疗两部分.预防部分把实验动物无特定致病菌C57BL/6小白鼠分成4组,分别通过灌胃方法给予AB(100μg)加CT(2 μg)、生理盐水、单纯AB(1 00 μg)、单纯CT(2 μg),1次/wk,共4次. 2 wk后再用活H pylori灌胃,再4 wk后处死动物.治疗部分把已感染H pylori的小白鼠分成4组,分组与治疗方法同预防部分,治疗结束后4 wk处死动物.取胃黏膜行半定量细菌培养检查H pylori情况. 结果:预防实验的保护率分别为AB加CT61.5%(16/26), 单纯AB、单纯CT和PBS保护率均为0.AB加CT治疗组H pylori根除率为:38.5%(1 0/26),生理盐水组、单纯AB、单纯CT组均无治疗作用.治疗组未根除H pylori的小鼠,疫苗组H pylori的定植密度明显低于其他4组(P<0.05). 结论:由AB加免疫佐剂组成的口服疫苗,不仅有预防H pylori感染的作用,同时也有根除已感染的H pylori 的作用.AB可用于H pylori疫苗的研制.
OBJECTIVE: To study the role of AB in the prevention and treatment of H pylori infection in mice infected with human H pylori.Methods: The experiment was divided into two parts: prevention and treatment, Animals were divided into 4 groups according to the pathogen-free pathogen C57BL / 6. The animals were given AB (100μg) plus CT (2μg), normal saline, AB (100μg), CT 1 times / wk, a total of 4 times .2 wk and then live H pylori gavage, and then 4 wk after the animals were killed .HPyl infection has been treated mice were divided into 4 groups, grouping and treatment with the prevention part of the treatment Animals were sacrificed 4 wk after the end of the experiment.Perivial cultures of gastric mucosa were examined for H pylori.Results: The protective rates of prophylaxis were AB plus CT61.5% (16/26), AB, CT and PBS alone Were 0. AB plus CT treatment of H pylori eradication rate was: 38.5% (10/26), saline group, simple AB, CT group were not treated alone treatment group did not eradicate H pylori mice, the vaccine Group H pylori colonization density was significantly lower than the other 4 groups (P <0.05) .Conclusion: The oral vaccine consisting of AB plus immune adjuvant, not only to prevent H pyl ori infection, but also eradicated the role of H pylori infection.AB can be used for the development of H pylori vaccine.