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RAR1和SGT1是植物R基因(抗病基因,resistance genes)介导的抗病防卫途径中的两个重要信号元件,在R蛋白下游和活性氧产生之间发挥作用。为了研究湖北海棠MhRAR1和MhSGT1基因在感病苹果品种植株内的表达特性及其抗病功能,利用农杆菌介导法将两种基因分别转入富士苹果。对Hyg阳性植株进行PCR和RT-PCR检测,6个转MhRAR1株系和3个转MhSGT1株系呈阳性;两种转基因苹果株系被苹果轮纹病病原菌侵染后,与对照相比都表现出抗氧化酶(SOD/POD/CAT)活性迅速升高、MDA含量变化较平缓的趋势,进一步说明RAR1和SGT1基因能够作用于苹果体内的活性氧反应和细胞膜活动进程,对植株受到外部侵害后的抗性反应有促进作用。
RAR1 and SGT1 are two important signaling elements in the disease-resistant defense pathway mediated by plant R genes that play a role downstream of R proteins and reactive oxygen species production. In order to study the expression characteristics and disease resistance of MhRAR1 and MhSGT1 genes in susceptible apple cultivars, the two genes were transferred into Fuji apple by Agrobacterium-mediated method. Hyg positive plants were tested by PCR and RT-PCR, 6 transgenic MhRAR1 strains and 3 transgenic MhSGT1 strains were positive; two transgenic apple lines infected by the pathogen of apple ring disease, compared with the control The activity of SOD / POD / CAT increased rapidly and the content of MDA changed gently. It further indicated that RAR1 and SGT1 could act on reactive oxygen species reaction and cell membrane activity in apple. The resistance reaction has a promoting effect.