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作者等对具有抗癌作用的中药,采用以生物试验为指标的方法,先将分离有效成分进行纯化,然后再运用各种物理化学的方法,确定其化学结构、进行合成结构修饰,制备衍生物,以阐明结构与抗癌作用之关系,最后再研究作用机理。其中所使用之生物试验,包括NCI 推行的体外KB 细胞法和体内小鼠P388白血病抗癌作用两种筛选法,经此筛得有效成分,如对后者(P388)显示极高效果(%T/C)时,则可再进行其对L1210、B16黑色素瘤、Lewis 肺癌以及结肠癌与乳腺癌等的抗癌试验,以确定其抗
Authors such as anti-cancer effect of traditional Chinese medicine, biological testing as an indicator of the method, the first separation of active ingredients to be purified, and then use a variety of physical and chemical methods to determine its chemical structure, the synthesis of structural modifications, the preparation of derivatives , To clarify the relationship between structure and anti-cancer effect, and finally study the mechanism of action. The biological assays used include two screening methods: the in vitro KB cell method by NCI and the anti-cancer effect of mouse P388 leukemia in vivo, after which the active ingredients are screened such as the P388 shows extremely high efficacy (% T / C), its anticancer test on L1210, B16 melanoma, Lewis lung cancer, and colon and breast cancer, etc., may be further conducted to determine its anti-cancer