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[目的]研究CYP2E1RsaⅠ、GSTT1、GSTM1基因多态性和烟酒茶嗜好及其相互作用与食管癌易感性的关系。[方法]在食管癌高发区淮安市进行了一个病例对照研究(食管癌144例 ,人群对照233例) ,调查研究对象的烟酒茶嗜好习惯 ,以PCR扩增、RsaⅠ内切酶消化 ,分析CYP2E1的基因型 ,以多重PCR方法分析GSTT1、GSTM1基因型。[结果](1)对照组GSTM1基因型频度及饮茶状况分布与食管癌组之间存在显著差异 ,携带GSTM1正常基因型或有饮茶习惯者发生食管癌的危险性显著降低。(2)在不同烟酒茶嗜好者中 ,CYP2E1、GSTT1和GSTM1基因型对食管癌发生的影响有所不同。[结论](1)携带GSTM1正常基因型或饮茶能降低食管癌发生的危险性。(2)在食管癌发生中存在环境因素与遗传因素的相互作用
[Objective] To study the relationship between polymorphisms of CYP2E1RsaI, GSTT1, and GSTM1 genes and tea and tea taste and their interaction with susceptibility to esophageal cancer. [Methods] A case-control study (144 cases of esophageal cancer and 233 control subjects) was conducted in Huai’an City, a high-incidence area of esophageal cancer. The habits of tobacco, alcoholic tea and tea were investigated, and the samples were digested by PCR amplification and RsaI endonuclease digestion. The genotypes of CYP2E1 were analyzed by multiplex PCR for the GSTT1 and GSTM1 genotypes. [Results] (1) There was a significant difference between the frequency of GSTM1 genotype distribution and the distribution of drinking tea in the control group and the esophageal cancer group. The risk of developing esophageal cancer was significantly lower in those carrying the GSTM1 normal genotype or tea drinking habits. (2) CYP2E1, GSTT1, and GSTM1 genotypes have different effects on the occurrence of esophageal cancer in different tea, tea and alcohol addicts. [Conclusion] (1) Carrying the normal genotype of GSTM1 or drinking tea can reduce the risk of esophageal cancer. (2) There is an interaction between environmental factors and genetic factors in the development of esophageal cancer