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目的:考察蛙凝素(Odorranalectin,OL)修饰对聚谷氨酸苄酯-聚乙二醇纳米粒(PBLG-PEG-NPs)材料的Calu-3细胞(人肺腺癌细胞)毒性和细胞摄取作用的影响。方法:碘氧化法制备蛙凝素修饰聚合物材料;以姜黄素(curcumin,Cur)为模型药物,采用乳化溶媒蒸发法制备聚谷氨酸苄酯-聚乙二醇纳米粒(PBLG-PEG-NPs)和蛙凝素修饰聚谷氨酸苄酯-聚乙二醇纳米粒(OL-PBLG-PEG-NPs);MTT法考察三种纳米粒对Calu-3的细胞毒性;采用激光共聚焦显微镜对两种纳米粒的Calu-3细胞摄取作用进行定性观察。结果:给予高浓度(2 mg·mL-1)纳米粒培养Calu-3细胞时,细胞存活率大于75%。蛙凝素修饰纳米粒后其被细胞摄取的量从62.7%增加到了81.2%。结论:OL可用于黏膜给药载体的修饰,OL-PBLG-PEG-NPs细胞毒性低、生物相容性好,有望成为一种鼻腔粘膜给药优良载体。
OBJECTIVE: To investigate the toxicity and cellular uptake of Calu-3 cells (human lung adenocarcinoma cells) by polyglutamic acid benzyl ester-polyethylene glycol nanoparticles (PBLG-PEG-NPs) modified by Odorranalectin (OL) Effect of the effect. Methods: Rhodamine-modified polymer was prepared by iodine oxidation. Curcumin (Cur) was used as model drug to prepare poly (L-glutamic acid) (NP-NPs) and polyglutamic acid benzyl ester-polyethylene glycol nanoparticles (OL-PBLG-PEG-NPs) were modified by rutin. MTT assay was used to examine the cytotoxicity of three nanoparticles on Calu-3. Laser confocal microscopy Qualitative observation of the uptake of Calu-3 cells by two kinds of nanoparticles was performed. Results: The cell survival rate of Calu-3 cells cultured in high concentration (2 mg · mL-1) nanoparticles was higher than 75%. The amount of cellular uptake by rodenticide-modified nanoparticles increased from 62.7% to 81.2%. CONCLUSION: OL can be used to modify mucosal drug delivery carriers. OL-PBLG-PEG-NPs have low cytotoxicity and good biocompatibility and are expected to be a good carrier for nasal mucosa delivery.