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自东北农学院育种试验地的马铃薯植株上所分离的14个具有卷叶症状的病毒标样经生物鉴定、蚜传特性、粒体形态和血清反应等鉴定均为PLRV。分离物85-1在曼陀罗上繁殖,经PEG沉淀,两次差速离心和蔗糖梯度离心后的得毒率为0.1mg/kg材料。提纯材料以接种后4～6周的曼陀罗最好。
Fourteen virus samples with leaf curl symptom isolated from potato plants in northeast agronomical breeding trials were identified by bioassay, and the characteristics of aphids, mitochondria and serum were identified as PLRV. Isolate 85-1 was propagated on Datura stramonium, PEG-precipitated, centrifuged twice and centrifuged sucrose at a rate of 0.1 mg / kg. Purified material 4 to 6 weeks after inoculation Datura best.