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本文报道运用巢式PCR,ELISA等方法检测我国原发性肝癌,癌旁组织和外周血中HCV-RNA抗-HCV,同时监测了HBsAg。所用引物位于HCV基因5’-端非编码区域。结果76例肝癌病人血清中抗-HCV阳性9.2％(7/76),HCV-RNA阳性13.2％(10/76);34例肝癌组织中HCV-RNA阳性11.8％(4/34),1例抗HCV阴性的病人在癌及癌旁组织中检测到ECV-RNA,提示我国HCC的发生与HCV感染有一定关系。本组检测10例HCV-RNA阳性,其中4例(40％)系第二步扩增后检出,说明巢式PCR确可显著提高HCV-RNA检出率,单纯用C100-3抗体检测有可能低估HCC中HCV的感染率。本研究还发现HCV-RNA阳性的HCC中HBsAg检出率高于HCV-RNA阴性HCC者,(P<0.05),说明在我国HCC的发生中,HCV可单独存在,但更常与HBV共同作用。
This article reported the use of nested PCR, ELISA and other methods to detect HCV-RNA anti-HCV in primary liver cancer, adjacent tissue and peripheral blood, and to monitor HBsAg. The primers used were located in the 5’-end non-coding region of the HCV gene. Results Anti-HCV positive was 9.2% (7/76) in serum, and HCV-RNA was positive in 13.2% (10/76) in 76 patients with liver cancer. HCV-RNA was positive in 11.8% (34/76) in 34 cases of liver cancer. Anti-HCV negative patients detected ECV-RNA in cancer and adjacent tissues, suggesting that the occurrence of HCC in China has a certain relationship with HCV infection. In this group, 10 cases of HCV-RNA were detected, of which 4 cases (40%) were detected after the second step of amplification, indicating that nested PCR can significantly improve the detection rate of HCV-RNA, and detection with C100-3 antibody alone The HCV infection rate in HCC may be underestimated. This study also found that the detection rate of HBsAg in HCV-RNA-positive HCC was higher than that of HCV-RNA-negative HCC (P<0.05), indicating that in the occurrence of HCC in China, HCV can exist alone, but it is more commonly associated with HBV. .