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本文介绍在精密pH电位法测量中用MAGEc计算机程序对玻璃电极进行选择和校正的方法.将该程序和MINIQUAD-82A联合使用,精确测定了L-半胱氨酸在生理条件下(37℃,I=150mmol/L Nacl)的质子化常数:lgβ_(11)=10.149±0.007,lgβ_(12)=18.132±0.012,lgβ_(13)=20.169±0.015.
This article presents a method for selecting and calibrating the glass electrode using the MAGEc computer program in the measurement of the precision pH potentiometry.The program was used in conjunction with the MINIQUAD-82A to accurately determine the L-cysteine content under physiological conditions (37 ° C, I = 150mmol / L Nacl) protonation constants: lgβ_ (11) = 10.149 ± 0.007, lgβ_ (12) = 18.132 ± 0.012, lgβ_ (13) = 20.169 ± 0.015.