论文部分内容阅读
该研究旨在探讨Sp1抑制剂光神霉素A(Mithramycin A)对人肺腺癌A549/DDP细胞MRP1表达的影响。不同浓度光神霉素A作用A549/DDP细胞48 h后,采用MTT法检测细胞存活率,Real time RT-PCR检测Sp1和MRP1基因表达水平,Western blot检测Sp1和MRP1蛋白表达水平。结果显示,300 nmol/L光神霉素A作用A549/DDP细胞48 h后Sp1和MRP1 mRNA表达水平分别降低31.22%和85.44%,Sp1和MRP1蛋白表达水平分别降低53.27%和40.42%。提示光神霉素A能够通过抑制Sp1表达,从而抑制MRP1表达。
This study aimed to investigate the effect of Mithramycin A, a Sp1 inhibitor, on MRP1 expression in human lung adenocarcinoma A549 / DDP cells. Cell viability was detected by MTT assay after transfected A549 / DDP cells with different concentration of mithramycin A for 48 h. Sp1 and MRP1 gene expression levels were detected by Real time RT-PCR and Sp1 and MRP1 protein levels by Western blot. The results showed that the expression of Sp1 and MRP1 mRNA decreased by 31.22% and 85.44% respectively after treated with 300 nmol / L mithramycin A for 48 h, while the expression levels of Sp1 and MRP1 decreased by 53.27% and 40.42%, respectively. It is suggested that mithramycin A can inhibit MRP1 expression by inhibiting Sp1 expression.