论文部分内容阅读
Aim:To determine whether deguelin can regulate the expression of nuclear factorkappa B(NF-κB)binding protein(IκBα)in U937 human leukemia cells and Rajihuman B lymphoma cells.Methods:The localization of IκBα protein was investi-gated by using an immunofluorescence method.The expression of IκBα and NF-κB/p65 proteins in Raji and U937 cells were investigated by using Western blotting.Apoptosis was detected through annexin V/PI double-labeled cytometry.Results:IκBα localized in the cytoplasm in untreated and deguelin-treated cells.Aftertreatment with tumor necrosis factor α(TNF-α)or deguelin plus TNF-α for 15 min,there was a substantial reduction in the amount of IκBα protein.The expressionof IκBα was downregulated by deguelin in Raji and U937 cells.Deguelin inducedapoptosis in U937 cells.Conclusion:Deguelin inhibited the expression of IκBαprotein in U937 and Raji cells.The anti-proliferative activity of deguelin is relatedto the signal pathway of NF-κB.
Aim: To determine whether deguelin can regulate the expression of nuclear factorkappa B (IκBα) in U937 human leukemia cells and Rajihuman B lymphoma cells. Methods: The localization of IκBα protein was instisti-gated by using an immunofluorescence method. The expression of IκBα and NF-κB / p65 proteins in Raji and U937 cells were investigated by using Western blotting. Apoptosis was detected by annexin V / PI double-labeled cytometry. Results: IκBα localized in the cytoplasm in untreated and deguelin- treated cells. Aftertreatment with tumor necrosis factor α (TNF-α) or deguelin plus TNF-α for 15 min, there was a substantial reduction in the amount of IκBα protein. expression of IκBα was downregulated by deguelin in Raji and U937 cells. Deguelin inducedapoptosis in U937 cells. Confluence: Deguelin inhibited the expression of IκBαprotein in U937 and Raji cells. The anti-proliferative activity of deguelin is relatedto the signal pathway of NF-κB.