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电镜形态学观察是肾小球疾病诊断、研究的重要方法。但仅根据形态观察结果常常难以肯定肾小球超微结构损伤的免疫病理学性质。随着肾活检的日益广泛开展,探索能够与常规电镜检查相结合,简便、可靠的肾活检免疫电镜检查方法,在电镜水平上明确超微结构改变与免疫病理损伤之间的关系,已成为肾脏病学家与电镜工作者共同关心的问题。为此,我们以临床肾活检标本为材料,采用胶体金免疫电镜技术,对肾小球免疫球蛋白成分及纤维蛋白原相关抗原(FRA)的超微结构进行定位检测,从而探讨该项技术用于肾小球疾病诊断与研究的意义、价值及与常规电镜检查相结合的可行
Electron microscopy morphological observation of glomerular disease diagnosis, an important method of study. However, it is often difficult to confirm the immunopathological properties of glomerular ultrastructure damage based only on morphological observations. With the development of renal biopsy, exploring the simple and reliable renal biopsy immunohistochemistry combined with routine electron microscopy to clarify the relationship between ultrastructural changes and immunopathological damage at the level of electron microscope, The problem of common concern between sick and electroscope workers. To this end, we use clinical renal biopsy specimens as materials, using the colloidal gold immunoelectron microscopy to detect the glomerular immunoglobulin components and the ultrastructure of fibrinogen-related antigen (FRA), so as to explore the technology The significance and value of glomerular disease diagnosis and research and the feasibility of combining with conventional electron microscopy