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目的:为了了解胎肝干细胞是否具有向神经组织细胞分化的潜能。方法:PCR检测sry基因分析孕14.5d胚胎鼠性别;采用免疫磁珠法分离雄性胎鼠肝的Sca-1~+细胞,尾静脉注射Sca-1~+细胞(2.0×10~3个/小鼠)到致死剂量放射线照射的雌性小鼠。移植60、120、180 d后采用免疫组化和FISH双染色检测受体小鼠脑组织中供体来源细胞特性。结果:受体雌鼠脑组织内存在大最Y染色体阳性细胞。免疫组化分析显示,部分Y染色体阳性细胞表达神经组织特异标志,如神经元核特异蛋白(NeuN,Neuron-specific nuclei protein)、部分Y染色体阳性细胞表达星形胶质细胞特异标志,如胶质纤维酸性蛋白(GFAP,Glial fibrillary acidic protein)等。结论:移植的胎肝Sca-1~+细胞,含造血干细胞,能分化成神经细胞和星形胶质细胞。
Objective: To understand whether fetal liver stem cells have the potential to differentiate into neural tissue cells. Methods: The sry gene was analyzed by PCR. The Sca-1 ~ + cells of male fetal rat liver were isolated by immunomagnetic beads method. Sca-1 ~ + cells (2.0 × 10 ~ 3 cells / Mouse) to lethal dose radiation irradiated female mice. After transplanted for 60, 120 and 180 days, the characteristics of donor-derived cells in recipient mouse brain were detected by immunohistochemistry and FISH double staining. Results: There were large Y chromosome positive cells in the brain of recipient mice. Immunohistochemical analysis showed that some Y chromosome-positive cells expressed neural tissue-specific markers such as Neuron-specific nuclei protein (NeuN) and some Y chromosome-positive cells expressed astrocyte-specific markers such as glial Glial fibrillary acidic protein (GFAP) and the like. Conclusion: Transplanted fetal liver Sca-1 ~ + cells, including hematopoietic stem cells, can differentiate into neurons and astrocytes.