Immobilization of laccase by Cu~(2+) chelate affinity interaction on surface modified magnetic silic

• 出 处: Chinese Chemical Letters
• 发表日期: 2012年02期
• 关键词: laccase silica chelate chelating polyacrylamide immobilization affinity grafted

Magnetic Cu~(2+)-chelated silica particles using polyacrylamide as a metal-chelating ligand was developed and used for the immobilization of laccase by coordination.The effect of pH and temperature on the enzymatic property of immobilized laccase and its catalytic capacity for pentachlorophenol(PCP) degradation were evaluated systemically.Compared with free laccase,the immobilized laccase showed improved acid adaptabihty and thermal stability.The immobilized laccase prepared in this work exhibited a good catalytic capacity for PCP removal from aqueous solutions.

Magnetic Cu ~ (2 +) - chelated silica particles using polyacrylamide as a metal-chelating ligand was developed and used for the immobilization of laccase by coordination. The effect of pH and temperature on the enzymatic property of immobilized laccase and its catalytic capacity for pentachlorophenol (PCP) degradation were evaluated systemically. Compared with free laccase, the immobilized laccase showed improved acid adaptabihty and thermal stability. Immobilized laccase prepared in this work exhibited a good catalytic capacity for PCP removal from aqueous solutions.