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对一个典型的籼/粳组合、窄叶青8号/京系17的F_1花药进行培养,所获二倍体花粉植株除约20％自然结实率为0外,其余植株的结实率在1%至正常结实之间,65％株系在75％以上,并稳定地传至自交后代。对130多个加倍单倍体（DH）多种性状的遗传进行了研究与分析,结果表明,花药培养可以克服籼/粳后代包括育性在内的各种性状长期分离不易稳定的严重障碍。生育期、株高、每穗粒数及千粒重等数量性状均为连续分布,呈正态曲线,为多基因控制。涉及籼与粳分类的一些性状,如叶毛、稃毛、酚反应、落粒性等的遗传,有的为一对主基因控制,有的为多基因决定。根据程氏形态指数对各个DH系的籼、粳属性进行分类,可以看到除籼型与粳型株系各占14％外,其余为各种偏籼和偏粳类型,形态指数也基本上呈正态分布。两个亲本均为早、中熟品种,但光温反应有所不同。DH群体对光温反应是多种多样的,既有不感光也不感温类型,也有对光温反应均较强类型等。各种性状都出现超亲分离,表明亚种间杂种的性状遗传存在大量多基因累加和互补作用。同时对籼/粳杂种不育的原因与产生的机理进行了讨论。
A typical indica / japonica combination, Fanyingqing 8 / Beijing 17 F_1 anther culture, the diploid pollen plants obtained except about 20% of the natural seed setting rate of 0, the rest of the seed setting rate of 1% Between the normal and strong, 65% of the lines are above 75%, and they are stably transmitted to the selfed offspring. The genetic analysis of more than 130 doubled haploid (DH) traits was conducted. The results showed that anther culture could overcome the serious obstacle of long-term separation of indica / indica offspring including fertility, which could not be easily stabilized by long-term isolation. Growth traits, plant height, grain number per spike and grain weight per thousand were all in continuous distribution with normal curve and multi-gene control. Some traits related to the indica and japonica classification, such as leaf hair, vellus hair, phenol reaction, shattering and other genetic, and some for a pair of major genes control, and some for multi-gene decisions. According to Cheng’s morphological index, the indica and japonica properties of each DH line were classified. It can be seen that except for 14% of indica and japonica lines, the others were all indica and japonica type, and the morphological index was basically Normal distribution. Both parents were early, middle-maturing varieties, but different light and temperature responses. DH population is a wide range of responses to light and temperature, both the type of light is not sensitive nor temperature, but also have a strong light and temperature response type. All traits showed super-pro-segregation, indicating that there is a large number of gene accumulation and complementarity between the traits of inter-subspecies hybrids. At the same time, the reasons and mechanism of hybrid sterility in indica / japonica rice were discussed.