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近年来,随着中药质量检验工作和现代检测分析技术的发展,中药鉴定除继续使用传统经验鉴定、原动(植)物鉴定和显微鉴定等方法之外,如色谱、光谱、电镜、免疫及生物试验等技术逐渐引入,解决了不少形态组织学方法难以鉴别的问题。为了适应基层加强药物分析和质量检测工作及培训中药检验鉴定技术人员的需要,兹对中药质量鉴定新技术作一简介,供作参考。一、色谱技术自1906年茨维特成功地用吸附柱分离植物色素以来,色谱技术迅速发展。柱层析(CC)、纸层析(PC)和薄层层析(TLC)已成为各级药检部门和中药研究机构日益广泛使用的方法,特别是薄层层析法已较为普及。1982年在太原召开的全国中药材薄层层析鉴别科
In recent years, along with the development of quality inspection of traditional Chinese medicines and the development of modern detection and analysis technologies, in addition to traditional methods of identification, original animal identification, and microscopic identification, such as chromatography, spectroscopy, electron microscopy, and immunoassay The introduction of technologies such as biological tests has been gradually introduced, which has solved a number of morphological histological methods that are difficult to identify. In order to adapt to the needs of the grass-roots level for drug analysis and quality testing and to train the needs of Chinese medicine inspection and appraisal technicians, we will provide a brief introduction to the new technologies for quality appraisal of Chinese medicine for reference. I. Chromatographic techniques Since 1906 Zwitte succeeded in separating plant pigments using adsorption columns, chromatographic technology has developed rapidly. Column chromatography (CC), paper chromatography (PC) and thin-layer chromatography (TLC) have become increasingly widely used methods at all levels of drug testing departments and traditional Chinese medicine research institutions. In particular, thin-layer chromatography has become more popular. National TLC Thin-Layer Chromatography Section, Taiyuan, China, 1982