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测定乌头碱类总碱的常用方法是中和法,但该法灵敏度低,用于含量较低的样品特别是乌头、附子制品中总碱的测定,往往需要消耗大量制品,其准确性也较差。为选择更有效的测定方法,我们对酸性染料萃取光度法用于乌头总碱的含量测定进行了比较系统的研究。实验发现甲基橙和次乌头碱等在PH5.0的缓冲液中可形成稳定的1:1络合物,经氯仿萃取后,在420nm处有一最大吸收。乌头碱、次乌头碱浓度在5—25ug/ml范围内符合Beer定律。据此建立的方法具有灵敏、精密、稳定等优点,为中草药及制剂中乌头碱总碱的测定提供了一种新的、有效的分析方法。
The commonly used method for the determination of aconitine alkaloids is the neutralization method. However, this method has a low sensitivity. It is used for the determination of total alkaloids in low-content samples, especially in aconite and aconite products. Also poor. In order to select a more effective method for the determination, we conducted a systematic study of the acid dye extraction spectrophotometric method for the determination of total alkaloids in Aconitum. It was found that methyl orange and hypaconitine could form a stable 1:1 complex in the buffer of pH 5.0. After being extracted by chloroform, there was a maximum absorption at 420 nm. The concentration of aconitine and hypaconitine in Beer’s law is in the range of 5-25 ug/ml. The established method has the advantages of sensitivity, precision and stability, and provides a new and effective analytical method for the determination of aconitine total base in Chinese herbal medicine and preparations.