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目的观察多氯联苯(PCB)-118对大鼠胰岛细胞INS-1的抑制作用。方法采用不同浓度PCB-118刺激INS-1细胞。MTT法检测INS-1细胞增殖率,实时荧光定量PCR检测胰岛素相关基因表达水平,以膜联蛋白Ⅴ-碘化丙啶(AnnexinⅤ-PI)双染和Western blot检测INS-1细胞凋亡及相关蛋白表达。结果采用80μmol/L和100μmol/L的PCB-118处理INS-1细胞72h后,INS-1细胞生长显著受到抑制(P<0.01)。与0μmol/L的PCB-118处理比较,50μmol/L的PCB-118处理INS-1细胞72h后,胰岛素基因1(Insulinl 1)、Insulinl 2、胰腺十二指肠同源框因子1(Pdx1)和葡萄糖转运子2(GT-2)的基因表达水平均下调(P<0.05或P<0.01),细胞凋亡率增高(P<0.05)。与0μmol/L的PCB-118处理比较,10μmol/L和50μmol/L的PCB-118处理细胞后,B淋巴细胞瘤-2(Bcl-2)蛋白表达降低,半胱氨酸天冬氨酸蛋白酶3(Caspase-3)剪切体和Bcl-2相关X蛋白(Bax蛋白)表达量增加,Bax/Bcl-2比值降低(P<0.05或P<0.01)。结论PCB-118可抑制INS-1细胞增殖,降低胰岛细胞功能,并诱导其凋亡;这提示PCB可能与糖尿病发病有关。
Objective To observe the inhibitory effect of polychlorinated biphenyls (PCBs) -118 on rat islet cells INS-1. Methods INS-1 cells were stimulated with different concentrations of PCB-118. The proliferation of INS-1 cells was detected by MTT assay. The expression of insulin-related genes was detected by real-time fluorescence quantitative PCR. The apoptosis of INS-1 cells was detected by AnnexinⅤ-PI double staining and Western blot. Protein. Results After INS-1 cells were treated with 80μmol / L and 100μmol / L PCB-118 for 72h, the growth of INS-1 cells was significantly inhibited (P <0.01). Compared with PCB-118 treated with 0μmol / L, INS-1 cells were treated with 50μmol / L PCB-118 for 72h, Insulin1 1, Insulin12, Pdx1 (P <0.05 or P <0.01) and the apoptosis rate of GT-2 cells were significantly increased (P <0.05). Compared with 0μmol / L PCB-118 treatment, the expression of Bcl-2 protein decreased after treated with 10μmol / L and 50μmol / L PCB-118, while caspase 3 (Caspase-3) and Bcl-2 related protein B (Bax protein) increased, and the ratio of Bax / Bcl-2 decreased (P <0.05 or P <0.01). Conclusion PCB-118 can inhibit the proliferation of INS-1 cells, reduce the function of islet cells and induce the apoptosis of INS-1 cells. This suggests that PCB may be involved in the pathogenesis of diabetes.