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目的进一步探讨微小病变型肾病综合征（ＭＣＮＳ）的发病有否ＴＨ１／ＴＨ２反应失衡。方法通过逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）和双抗体夹心ＥＬＩＳＡ法，检测１１例激素敏感型肾病综合征患儿外周血单个核细胞（ＰＢＭＣ）淋巴因子基因表达和蛋白产生。结果与正常对照组相比，ＮＳ患儿ＩＬ－１０、ＩＬ－４基因表达和蛋白产生明显增高、ＩＬ－２、ＩＬ－６降低，干扰素（ＩＦＮ）－γ和ＩＬ－５无明显差异。结论激素敏感性ＮＳ患儿ＴＨ１类和ＴＨ２类淋巴因子产生有重叠，不能用单一的ＴＨ１或ＴＨ２亚群过度活化解释，其淋巴因子产生异常可能是某些ＴＨ细胞克隆活化异常所致
Objective To investigate whether there is an imbalance of TH1 / TH2 response in the pathogenesis of minimal nephrotic syndrome (MCNS). Methods Lymphokine gene expression and protein production in peripheral blood mononuclear cells (PBMCs) from 11 children with steroid-induced nephrotic syndrome were detected by reverse transcription-polymerase chain reaction (RT-PCR) and double antibody sandwich ELISA. Results Compared with the normal control group, the expression of IL-10 and IL-4 mRNA and protein in NS children were significantly increased, while the levels of IL-2 and IL-6 were decreased. There was no significant difference in the levels of IFN-γ and IL-5. Conclusions There is overlap between TH1 and TH2 lymphokines in hormone-sensitive NS children and can not be explained by overexpression of a single TH1 or TH2 subpopulation. Abnormal lymphokine production may be caused by the abnormal activation of some TH cell clones