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目的明确三带喙库蚊对溴氰菊酯的抗药性及探讨吸血对抗药性的影响,以指导蚊虫抗药性的准确测定和化学杀虫剂的正确选择。方法从位于中缅边境地区的云南省德宏州盈江县现场采集吸血蚊和未吸血三带喙库蚊,采回后的第1d和第3d上午,应用WHO推荐的成蚊滤纸接触法分别测定其抗药性。结果在第1d和第3d的抗性测试中,未吸血蚊的死亡率分别是27.91%和28.15%。吸血蚊的首只击倒时间、KT50和KT95均大于相应未吸血蚊;击倒率和死亡率都小于相应未吸血蚊(击倒率,χ2=4.27,P=0.04<0.05&χ2=5.4,P=0.02<0.05;死亡率,χ2=13.28,P=0.0004<0.05&χ2=8.88,P=0.0029<0.05)。结论云南省盈江县的三带喙库蚊已对溴氰菊酯产生很强的抗药性,吸血可以显著增强该抗药性。因此,在蚊虫的抗药性测定中,要尽量排除吸血的影响,以获得准确地蚊虫抗药性水平,指导杀虫剂的合理使用。
Objective To determine the resistance of Culex trituberculatum to deltamethrin and to explore the influence of blood-sucking on its resistance to guide the accurate determination of insecticide resistance and the correct choice of chemical insecticides. Methods The mosquitoes and uninfected Culex tritaeniorhynchus collected from Yingjiang County, Dehong Prefecture, located in the border between China and Myanmar. The mosquito filter paper contact method recommended by WHO was used on the 1st and 3rd morning after the recovery Determination of its resistance. Results The mortality of non-blood-sucking mosquitoes was 27.91% and 28.15% respectively in the first and third days of resistance testing. The first knock-down time of M. mosquito, KT50 and KT95 were greater than that of the corresponding non-absorbed mosquitoes; the knock-out rate and mortality were both less than those of the corresponding non-absorbed mosquitoes (knockdown rate, χ2 = 4.27, P = 0.04 <0.05 & χ2 = 5.4, P = 0.02 <0.05; mortality, χ2 = 13.28, P = 0.0004 <0.05 & χ2 = 8.88, P = 0.0029 <0.05). Conclusion Culex tritaeniorhynchus in Yingjiang County of Yunnan Province has strong resistance to deltamethrin, and vampus can significantly enhance its resistance. Therefore, in the determination of resistance of mosquitoes, we should try to exclude the impact of vampire in order to obtain accurate resistance levels of mosquitoes and guide the rational use of insecticides.