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观察70例患者(包括60例针麻及10例药麻),在针(或药)麻前及诱导20分钟后各自患者耳垂取血一次,部分患者在针刺24小时后又取血一次。电针主穴为合谷或足三里。作为体外细胞免疫检测方法我们采用改进的微量全血技术做玫瑰花(包括活性、非活性)及淋巴细胞转化试验。在做活性玫瑰花试验的同时,计数白细胞总数及淋巴细胞分类百分数以计算活性玫瑰花形成细胞(RFC)的绝对值。结果见到在活性RFC中针刺诱导后提高均值为12.7±1.43,降低均值为6.8±1.77。活性RFC绝对值的提高均值为175.33±63.59。然而药麻组的活性RFC在药麻后变动不明显。在淋巴细胞转化试验中,提高均值为12.7±1.29,降低均值为7.0±2.19。在针刺24小时后仍见提高效应。以上提高变动多见于针前细胞免疫水平偏低或一般者,而降低变动可见于针前水平偏高者。在活性、非活性玫瑰花及淋巴细胞转化三项试验中,提高或降低变动相符,提高约12～13％,降低约6～7％,两者相比皆以提高为主。
Observed in 70 patients (including 60 acupuncture and 10 cases of medicine hemp), before the needle (or medicine) before and 20 minutes after the induction of the patient’s earlobe to take blood once, some patients in the 24 hours after acupuncture blood. The main point of electro-acupuncture is Hegu or Zusanli. As an in vitro method for cell immunoassay, we used modified micro-whole blood technology for rose (including active and inactive) and lymphocyte transformation assays. While performing a reactive rose test, the total number of leukocytes and the percentage of lymphocyte fractionation were counted to calculate the absolute value of reactive rose forming cells (RFCs). The results showed that the mean increase in acupuncture induction in active RFC was 12.7±1.43 with a mean reduction of 6.8±1.77. The mean increase in the absolute value of active RFC was 175.33±63.59. However, the activity of RFC in the pharmacopoeia group did not change significantly after pharmacotherapy. In lymphocyte transformation assays, the mean increase was 12.7±1.29 and the mean reduction was 7.0±2.19. The improvement effect was still seen after 24 hours of acupuncture. The above changes were more common in the pre-needle cells with low or general cellular immunity levels, while the lower changes were seen in the pre-needle levels. In the three trials of active and inactive rose and lymphocyte transformation, the increase or decrease was consistent with the increase of about 12% to 13% and the decrease of about 6% to 7%, both of which were mainly improved.