论文部分内容阅读
目的通过检测基质金属蛋白酶(MMPs)在急性白血病(AL)和中枢神经系统白血病(CNSL)患儿脑脊液中的表达情况及患儿确诊CNSL前后脑脊液MMPs的动态表达情况,探讨MMPs与儿童AL发生发展和髓外浸润的关系。方法采用明胶酶谱活性分析方法和图像分析系统检测22例未发生CNSL的AL患儿、18例CNSL患儿和3例非肿瘤非感染性疾病患儿脑脊液MMP-2、MMP-9的活性表达,并且动态检测CNSL患儿确诊CNSL前后脑脊液MMP-2、MMP-9的活性变化。结果MMP-2和MMP-9在对照组中的表达率为0,酶解活性水平分别为67.15±16.6和23.55±10.08;MMP-2和MMP-9在未发生CNSL的AL组中的表达率为31.8%,酶解活性水平分别为115.13±43.67和60.86±37.80,显著高于对照组(Pa<0.05);而MMP-2和MMP-9在发生CNSL的AL组中的表达率为100%,酶解活性水平分别为867.55±308.67和562.88±139.22,显著高于未发生CNSL组和对照组(Pa<0.05)。MMPs过量表达,特别是MMP-2在CNSL患儿脑脊液中高表达与白血病细胞浸润高度相关。结论MMP-2和MMP-9在AL患儿脑脊液中异常表达,可能参与了儿童AL的发生发展;通过检测MMP-2和MMP-9可间接反映白血病中枢神经系统浸润的发生和发展情况,并可对高危人群早期加强治疗,从而预防髓外浸润的发生,对指导临床诊断和治疗具有重要意义。
Objective To investigate the expression of matrix metalloproteinases (MMPs) in cerebrospinal fluid of children with acute leukemia (AL) and central nervous system leukemia (CNSL) and the dynamic expression of MMPs in children with cerebrospinal fluid And extramedullary infiltration of the relationship. Methods The activity of MMP-2 and MMP-9 in cerebrospinal fluid of 22 AL children without CNSL, 18 CNSL children and 3 non-neoplastic non-infectious disease children were detected by gelatin zymography and image analysis system. , And dynamically detected the activity of MMP-2 and MMP-9 in cerebrospinal fluid in patients with CNSL before and after the diagnosis of CNSL. Results The positive expression rate of MMP-2 and MMP-9 in the control group was 0 and the enzymatic activity levels were 67.15 ± 16.6 and 23.55 ± 10.08, respectively. The expression rates of MMP-2 and MMP-9 in AL group without CNSL Was 31.8%, the enzymatic activity levels were 115.13 ± 43.67 and 60.86 ± 37.80, respectively, which were significantly higher than that of the control group (Pa <0.05); while the expression rates of MMP-2 and MMP-9 in AL group with CNSL were 100% , Respectively. The levels of enzymatic activity were 867.55 ± 308.67 and 562.88 ± 139.22, respectively, which were significantly higher than those without CNSL or control (Pa <0.05). Overexpression of MMPs, especially MMP-2 in cerebrospinal fluid of CNSL patients is highly correlated with leukemic cell infiltration. Conclusions MMP-2 and MMP-9 are abnormally expressed in cerebrospinal fluid of children with AL and may be involved in the development and progression of AL in children. The detection of MMP-2 and MMP-9 indirectly reflect the occurrence and development of central nervous system infiltration of leukemia Early treatment of high-risk groups can be strengthened to prevent the occurrence of extramedullary infiltration, to guide the clinical diagnosis and treatment of great significance.