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[目的]观察解毒化瘀方对轻微型肝性脑病大鼠行为学、学习记忆能力、血浆内毒素、血氨、肝功能及肝脏病理的影响。[方法]使用随机平行对照方法,将60只SD雄性大鼠编号按随机数字表法随机分为正常对照组A、模型组B、中药组C、乳果糖组D、中药+乳果糖组E(12只/组)。除正常对照组,各组皆予小剂量硫代乙酰胺(TAA)200mg/kg隔日腹腔注射建立MHE模型,造模前5日至造模后3天B、C、D、E组分别给予生理盐水、解毒化瘀汤剂、乳果糖、解毒化瘀汤剂+乳果糖灌胃10天。观察行为学、学习记忆能力变化,检测血浆内毒素、血氨、肝功能、肝组织病理变化。[结果]模型组大鼠学习和记忆功能下降,血浆内毒素、血氨、肝功等明显升高,出现肝细胞气球样变和中央静脉为中心的肝小叶点状坏死,炎性细胞浸润明显。中药组、乳果糖组、中药+乳果糖组大鼠学习和记忆功能、血浆内毒素、血氨、肝功、肝脏病理等较之明显好转(P<0.05);三者差异无显著性(P>0.05)。[结论]解毒化瘀方对TAA诱导的MHE大鼠有一定的保护作用。
[Objective] To observe the effects of Jiedu Huayu Recipe on behavior, learning and memory abilities, plasma endotoxin, blood ammonia, liver function and liver pathology in mild hepatic encephalopathy rats. [Methods] Using randomized parallel control method, 60 SD male rats were randomly divided into normal control group A, model group B, Chinese medicine group C, lactulose group D, TCM + lactulose group E ( 12 / group). In addition to the normal control group, MHE model was established by intraperitoneal injection of 200mg / kg TAA in low dose of TAA, B, C, D and E groups from 5 days before modeling to 3 days after modeling respectively. Salt water, detoxification and stasis decoction, lactulose, detoxification stasis decoction + lactulose intragastric administration of 10 days. Observation of behavior, learning and memory ability changes, detection of plasma endotoxin, blood ammonia, liver function, pathological changes of liver tissue. [Result] The learning and memory function of the model group decreased, the plasma endotoxin, blood ammonia, liver function and so on were obviously increased. The hepatocellular lobular necrosis and the infiltration of inflammatory cells were obvious . The learning and memory function, plasma endotoxin, blood ammonia, liver function and liver pathology were significantly improved (P <0.05) in the traditional Chinese medicine group, lactulose group and traditional Chinese medicine + lacto-lactose group. There was no significant difference among the three groups > 0.05). [Conclusion] Jiedu Huayu Recipe has certain protective effect on TAA-induced MHE rats.