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采用完全随机(添加处理5×发酵天数2)实验设计,对风干玉米秸秆进行无添加、乳酸菌(LAB)添加、酸液4%、6%和8%添加共计5个处理,发酵30 d和60 d开封。分析了p H、饲料化学成分、微生物组成及In vitro干物质消失率和甲烷生成量。经过发酵30及60 d的p H,LAB、4%、6%和8%的酸添加组均低于对照组,并且在相同添加组中,p H值随发酵时间的增加而显著下降。在饲料化学成分方面,所有添加组的有机物(OM)均高于对照组;LAB添加组的粗蛋白含量(CP)和粗脂肪(EE)均高于其他处理组。在微生物上,30或60 d的5个添加组的乳酸菌、耐热菌、一般细菌及酵母菌分别显著低于对照组;同时在每个处理中,各微生物60 d均低于30日。在In vitro干物质消失率和甲烷生成量方面,所有添加处理组的干物质消失率和甲烷生成量分别高于和低于对照组。以上结果表明,黄贮玉米秸秆的发酵品质60日好于30日,乳酸菌及混合酸处理玉米秸秆,均能提高黄贮玉米秸秆发酵品质,混合酸处理玉米秸秆能够不同程度降解秸秆中的粗纤维为非纤维性碳水化合物,乳酸菌和6%混合酸处理玉米秸秆可以提高In vitro干物质消失率,同时降低甲烷生成量。
A total of 5 treatments including 4%, 6% and 8% addition of acid were added to the air-dried corn stover without any addition of lactic acid bacteria (LAB) d Kaifeng. P H, feed chemical composition, microbial composition, In vitro dry matter loss and methane production were analyzed. The p H, LAB, 4%, 6% and 8% acid addition groups after 30 and 60 days of fermentation were lower than the control group, and p H value decreased significantly with the increase of fermentation time in the same addition group. In terms of chemical composition of feeds, the content of organic matter (OM) of all the added groups was higher than that of the control group. The contents of crude protein (CP) and crude fat (EE) of LAB addition group were higher than those of other treatments. In the microorganisms, the lactic acid bacteria, heat-resistant bacteria, common bacteria and yeast in the five addition groups at 30 or 60 days were significantly lower than those in the control group, and the microorganisms in each treatment were lower than 30 days after 60 days. In addition, the dry matter disappearance rate and methane production of the added treatment groups were higher and lower than that of the control group respectively, in terms of the in vitro dry matter disappearance rate and the methane production amount. The above results showed that the fermented quality of yellow stalk corn stalks was better than that on the thirtieth day. Lactic acid bacteria and mixed acid treatment of corn stover could improve the fermented quality of yellow stalk corn stalks. The mixed acid treatment of corn stalks could degrade crude fiber in stalks to different degrees Treatment of corn stover with non-fibrous carbohydrates, lactic acid bacteria and 6% mixed acid increased In vitro dry matter loss and reduced methane production.