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目的 研究蕲蛇酶、凝血酶与不同种属来源的纤维蛋白原的凝血作用的差异 ,以选择检定蕲蛇酶活力的最适底物。 方法 取不同浓度人凝血酶与人纤维蛋白原 (HFg)、牛纤维蛋白原 (BFg)及人血小板血浆 (PPP)反应 (试管法或用血液凝聚仪 ) ,分别记录初凝时间并求反应曲线的回归方程。蕲蛇酶也稀释成不同浓度依上法测定 ,并求回归方程。另以人凝血酶 (1 .2 5IU/ ml)、蕲蛇酶 (1 .2 5AU/ ml)与梯度浓度的 HFg反应并求最大反应速度。此外还测定了蕲蛇酶以家兔 PPP为底物的凝血活力和以 BAEE、TAME为底物的精氨酸酯酶活力。 结果 人凝血酶、蕲蛇酶对 HFg的反应速度均比 BFg快 (P <0 .0 5) ;丝氨酸蛋白酶抑制剂 PMSF和金属蛋白酶抑制剂 EDTA均能抑制蕲蛇酶的凝血活力 ,但后者不能抑制蕲蛇酶的精氨酸酯酶活力。 结论 HFg是蕲蛇酶活力检定较合适的底物
Objective To study the difference of coagulation between fibrinogen, thrombin and fibrinogen from different species to select the optimum substrate for the determination of the activity of Acutobin. Methods Different concentrations of human thrombin and human fibrinogen (HFg), bovine fibrinogen (BFg) and human platelet plasma (PPP) reaction (test tube or blood coagulation analyzer) were recorded initial setting time and the response curve Regression equation. Viper also diluted into different concentrations according to the determination of law, and seek regression equation. The other with human thrombin (1.25 IU / ml), viper enzyme (1 .2 5AU / ml) and gradient concentration of HFg reaction and seek the maximum reaction speed. In addition, the activity of coagulation activity of rhodopsin with rabbit PPP as substrate and arginine esterase with BAEE and TAME as substrates were measured. Results The reaction rate of human thrombin and viper was faster than that of BFg (P <0.05). Both serine protease inhibitor PMSF and metalloproteinase inhibitor EDTA inhibited the coagulation activity of viper, but the latter Arginine esterase activity can not be inhibited. Conclusion HFg is a suitable substrate for the detection of Acutobin activity