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目的探讨激活素A(ActivinA)及其ⅡA型受体(ActRⅡA)在刀豆蛋白A(ConA)诱导小鼠急性肝损伤时的表达及其作用。方法尾静脉注射ConA诱导小鼠急性肝损伤,测定血清转氨酶水平判断肝脏组织损伤程度,实时定量RT-PCR检测ActivinA及ActRⅡAmRNA转录水平;应用抗ActivinA和ActRⅡA抗体进行阻断试验,测定血清转氨酶水平,HE染色观察肝组织病理学变化。结果ConA诱导的急性肝损伤模型小鼠血清转氨酶水平明显升高,ActivinA及ActRⅡAmRNA转录水平也明显高于对照组;体内应用抗ActivinA和ActRⅡA抗体阻断ActivinA和ActRⅡA的作用,均可不同程度减轻肝损伤。结论ActivinA是介导ConA诱导小鼠急性肝损伤的重要致病因子,阻断ActivinA的表达或其作用途径,可能成为治疗肝损伤疾病的有效靶点。
Objective To investigate the expression and its role of Activin A and ActRⅡA in acute liver injury induced by Con A in mice. Methods Acute liver injury induced by ConA was induced via caudal vein in mice. Serum aminotransferase (ALT) levels were measured to determine the degree of hepatic tissue injury. Real-time quantitative RT-PCR was used to detect the transcriptional level of ActivinA and ActRⅡA mRNA. Anti-Activin A and ActRⅡA antibodies were used to block the serum ALT, Hepatic histopathological changes were observed by HE staining. Results The level of serum aminotransferase and the level of Activin A and ActRⅡA mRNA in Con A -induced acute liver injury model were significantly higher than those in control group. The anti-Activin A and ActRⅡA antibodies blocked the effects of Activin A and ActRⅡA in vivo, damage. Conclusion ActivinA is an important virulence factor mediating acute liver injury induced by ConA in mice. Blocking the expression of ActivinA or its pathway may be an effective target for the treatment of liver injury.