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证实了两个不同品种的冬小麦根系表面存在着氧化ＮＡＤＨ和还原Ｋ３Ｆｅ（ＣＮ）６的氧化还原活力。还原铁氰化物活力在ＰＨ５．５到８．５范围内随着ｐＨ值升高而增大，温度在１５℃到４５℃范围内随温度升高还原活力增强，４５℃达最高值，５５℃时活力急剧下降．阳离子对小麦根还原Ｋ３Ｆｅ（ＣＮ）６活力有促进作用，其顺序为Ｌａ（３＋）＞Ｃａ（２＋）、Ｍｇ（２＋）＞Ｋ＋、Ｎａ＋．陕合６号还原Ｋ３Ｆｅ（ＣＮ）。的Ｋｍ为０．８５ｍｍｏｌ／Ｌ，Ｖ（ｍａｘ）≌１６６．６ｎｍｏｌｇ（－１）ＦＷ·ｍｉｎ（－１）；郑引一号还原Ｋ３Ｆｅ（ＣＮ）６的Ｋｍ值是１．３３ｍｍｏｌ／Ｌ，Ｖｍａｘ≌２３８．０ｎｍｏｌ·ｇ（－１）ＦＷ·ｍｉｎ（－１）。
It was confirmed that the redox activities of oxidized NADH and reduced K3Fe (CN) 6 existed in the roots of two different varieties of winter wheat. The activity of reduced ferricyanide increased with the increase of pH within the range of pH5.5to8.5. The activity of reduced ferricyanide increased with the increase of temperature in the range of15 ℃ to45 ℃, reached the highest value at55 ℃, When the vitality dropped dramatically. Cations promoted the activity of K3Fe (CN) 6 in wheat roots, and the order was La (3 +)> Ca (2 +), Mg (2 +)> K +, Na +. Shaanxi co-6 to restore K3Fe (CN). The Km of K3Fe (CN) 6 reduced by 1.83 mmol / L, Vmax (max) ≌166.6 nmolg (-1) FW · min ≌238.0 nmol · g (-1) FW · min (-1).