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年龄26—27天的Wistar雌鼠用PMSG与HCG诱导成熟,同时分别注射3、6、9 mg消炎痛,分离卵巢颗粒细胞并离体培养,培液中的PA活力经~(125)Ⅰ-纤维蛋白降解法检测,均无被抑制现象(图1)。仅用PMSG激动大鼠的颗粒细胞离体培养于含HCG及不同浓度(10~(-8)mol/L—10~(-5)mol/L)的消炎痛培液内,各组PA活力均未出现明显变化(图2)。离体条件下PGE_2增强PMSG激动大鼠颗粒细胞PA活力的事实说明,消炎痛抑制实验未能显示前列腺素与PA的关系,可能因所用以诱导大鼠临近排卵的PMSG和HCG分别所含FSH与LH的高活力促使PA活力达到最高点,以致前列腺素对PA的影响被掩盖;PGF_(2α)对PA未显示任何作用(图4)。另外,PGE_2使仅接受PMSG大鼠颗粒细胞PA升高的事实(图3)提示,PGE_2有摹拟LH的作用;未经PMSG与HCG处理鼠的卵泡细胞的PA活力也受PGE_2的影响,说明它还有摹拟FSH的作用。
Wistar females aged 26-27 days were induced to mature with PMSG and HCG, and 3, 6 and 9 mg of indomethacin were injected respectively. Ovarian granulosa cells were isolated and cultured in vitro. The PA activity in the culture fluid was determined by ~(125)I-. No fibrin degradation was detected (Figure 1). PMSG-induced rat granulosa cells were cultured in vitro in indomethacin-containing culture fluid containing HCG and different concentrations (10-8 mol/L-10 mol/L). No significant changes have occurred (Figure 2). The fact that PGE 2 enhances PA activity in PMSG-affected rat granulosa cells in vitro suggests that the indomethacin inhibition test failed to show the relationship between prostaglandin and PA, which may be due to the FSH and PMG and the HCG that were used to induce ovulation in rats. The high vitality of LH promoted the peak of PA activity so that the effect of prostaglandin on PA was masked; PGF_(2α) did not show any effect on PA ( FIG. 4 ). In addition, the fact that PGE 2 only increased PA in PMSG rat granulosa cells (Fig. 3) suggests that PGE 2 mimics the effect of LH; the PA activity of follicle cells not treated with PMSG and HCG is also affected by PGE 2, indicating that There is also the role of FSH.