论文部分内容阅读
目的:分析BAFF分子在肾移植受者外周血淋巴细胞的表达情况以及初步分析其异常表达的生物学意义。方法:以来院随访的肾移植受者为研究对象,采集患者外周血,以EDTA-Na2抗凝。采用单色和多色标记的免疫荧光分析法,分析BAFF分子在外周血淋巴细胞的表达情况,同时检测淋巴细胞CD3、CD4、CD8、CD134、CD25和CD127的表达。结果:共分析了86位肾移植受者。BAFF分子在受者外周血淋巴细胞的表达率分别介于0.18%至76.97%之间。以15%为界将所有数据分成两组(表达率>15%组和表达率<15%组),对所获得的数据进行统计分析。在BAFF分子表达率>15%组其BAFF分子表达的平均值为36.91%;BAFF+细胞群的增加与外周血CD4+/CD8+比值、与循环中CD4+CD25+CD127-/low T细胞群等没有显著相关性;但是与循环中CD134+ T细胞群和CD4+CD134+ T细胞群的增加存在显著相关性(P<0.01和P<0.05),具有统计学意义。而BAFF表达率<15%组所检测的相关指标均没有统计学意义。结论:BAFF分子在部分肾移植受者外周血淋巴细胞的异常高表达可能与移植排斥反应相关。
OBJECTIVE: To analyze the expression of BAFF molecules in peripheral blood lymphocytes of renal allograft recipients and to analyze the biological significance of their abnormal expression. Methods: The renal transplant recipients who were hospitalized for follow-up were enrolled in this study. Peripheral blood was collected from the patients for anticoagulation with EDTA-Na2. Immunofluorescence analysis with monochromatic and multicolor markers was used to analyze the expression of BAFF in peripheral blood lymphocytes and the expressions of CD3, CD4, CD8, CD134, CD25 and CD127 in lymphocytes. Results: A total of 86 renal transplant recipients were analyzed. The expression rates of BAFF molecules in peripheral blood lymphocytes of recipients ranged from 0.18% to 76.97%. All data were divided into two groups (expression rate> 15% and expression rate <15%) at 15%, and the data obtained were statistically analyzed. The average expression of BAFF in BAFF was 36.91%. The ratio of BAFF + cells to peripheral blood CD4 + / CD8 + and circulating CD4 + CD25 + CD127- / low T cells were not significantly different However, there was a significant correlation between circulating CD134 + T cell population and CD4 + CD134 + T cell population (P <0.01 and P <0.05). The BAFF expression rate of <15% of the group detected the relevant indicators were not statistically significant. Conclusion: The abnormally high expression of BAFF molecules in peripheral blood lymphocytes of partial renal transplant recipients may be related to graft rejection.